Species delimitation in poorly known and diverse taxa is usually performed based on monolocus, DNA-­barcoding-­like approaches, while multilocus data are often used to test alternative species hypotheses in well-­studied groups. We combined both approaches to delimit species in the Xenuroturris/Iotyrris complex, a group of venomous marine gastropods from the Indo-P­ acific. First, COI sequences were analysed using three methods of species delimitation to propose primary species hypotheses. Second, RAD sequencing data were also obtained and a maximum-l­ikelihood phylogenetic tree produced. We tested the impact of the level of missing data on the robustness of the phylogenetic tree obtained with the RAD-­seq data. Alternative species partitions revealed with the COI data set were also tested using the RAD-­seq data and the Bayes factor species delimitation method. The congruence between the species hypotheses proposed with the mitochondrial nuclear data sets, together with the morphological variability of the shell and the radula and the distribution pattern, was used to turn the primary species hypotheses into secondary species hypotheses. Allopatric primary species hypotheses defined with the COI gene were interpreted to correspond to intraspecific structure. Most of the species are found sympatrically in the Philippines, and only one is confidently identified as a new species and described as Iotyrris conotaxis n. sp. The results obtained demonstrate the efficiency of the combined monolocus/multilocus approach to delimit species.

ATIMO VATAE, INHACA 2011, MAINBAZA, Restricted, PAPUA NIUGINI, SANTO 2006, TERRASSES

Transcriptome-based exon capture methods provide an approach to recover several hundred markers from genomic DNA, allowing for robust phylogenetic estimation at deep timescales. We applied this method to a highly diverse group of venomous marine snails, Conoidea, for which published phylogenetic trees remain mostly unresolved for the deeper nodes. We targeted 850 protein coding genes (678,322 bp) in ca. 120 samples, spanning all (except one) known families of Conoidea and a broad selection of non-Conoidea neogastropods. The capture was successful for most samples, although capture efficiency decreased when DNA libraries were of insufficient quality and/or quantity (dried samples or low starting DNA concentration) and when targeting the most divergent lineages. An average of 75.4% of proteins was recovered, and the resulting tree, reconstructed using both supermatrix (IQ-tree) and supertree (Astral-II, combined with the Weighted Statistical Binning method) approaches, are almost fully supported. A reconstructed fossil-calibrated tree dates the origin of Conoidea to the Lower Cretaceous. We provide descriptions for two new families. The phylogeny revealed in this study provides a robust framework to reinterpret changes in Conoidea anatomy through time. Finally, we used the phylogeny to test the impact of the venom gland and radular type on diversification rates. Our analyses revealed that repeated losses of the venom gland had no effect on diversification rates, while families with a breadth of radula types showed increases in diversification rates, thus suggesting that trophic ecology may have an impact on the evolution of Conoidea.

ATIMO VATAE, AURORA 2007, BIOPAPUA, CEAMARC-AA, CONCALIS, Restricted, DongSha 2014, EXBODI, GUYANE 2014, ILES DU SALUT, INHACA 2011, KARUBENTHOS 2012, KAVIENG 2014, MAINBAZA, NORFOLK 2, NanHai 2014, PANGLAO 2005, PAPUA NIUGINI, Restricted, SALOMONBOA 3, TAIWAN 2013, TERRASSES, Restricted

AURORA 2007, INHACA 2011, KAVIENG 2014, PANGLAO 2004, PAPUA NIUGINI, SANTO 2006